پاورپوینت Biotechnology and Genetic Engineering

پاورپوینت Biotechnology and Genetic Engineering

فرمت فایل دانلودی: .zip
فرمت فایل اصلی: PowerPoint (pptx) (قابل ویرایش و آماده پرینت)
تعداد صفحات: 36
حجم فایل: 980390
قیمت: 52000 تومان

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پاورپوینت Biotechnology and Genetic Engineering (pptx) 36 اسلاید

دسته بندی : پاورپوینت

نوع فایل : PowerPoint (.pptx) ( قابل ویرایش و آماده پرینت )

تعداد اسلاید: 36 اسلاید

قسمتی از متن PowerPoint (.pptx) :

Biotechnology and Genetic EngineeringRecombinant DNA and DNA ScienceBiotechnologyThe use of living cells to make products such as pharmaceuticals foods and beveragesThe use of organisms such as bacteria to protect the environmentThe use of DNA science for the production of products diagnostics and researchRecombinant DNAThe manipulation and combination of DNA from two sources Bacterial DNA + human gene for insulinPlant DNA + bacterial DNA - Agrobacterium tumefaciensMouse DNA + human DNA = transgenicRecombinationInsert a foreign gene into a host Plasmid ( for example exogenous DNA) into the bacterial cell – transformation or transfection-organism referred to as transgenic ( eukaryote ) or recombinant( prokaryote)Goal – To produce many copies ( clones) of a particular geneReporter gene – tags gene of interest – to identify the presence of a geneVectors Plasmids VirusesParticles ( DNA coated bullets)Exogenous DNACharacteristics of a VectorCan replicate independently in the host cell – contains an OriHas restriction sites in the vector- Polylinker cloning regionHas a reporter gene that will announce its presence in the host cellIs a small size in comparison to the host chromosome for ease of isolationRestriction Enzymes and VectorsCut Plasmid with restriction enzymeCut gene of interest with restriction enzymeSplice together gene of interest and vectorTools for Recombination Restriction enzymesRecombinant DNA